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Immunofluorescence staining of AQP3 and AQP7 in human sperm. Green immunolabeling of AQP3 (A,C) and AQP7 (B,D) at two different amplifications. Negative controls were carried out, without a primary antibody, for each protein. Cell nuclei were stained blue with VECTASHIELD® Antifade Mounting Medium with DAPI. (A,B) White bar, 10 μm; (C,D) white bar, 50 μm.
