Table 7.
Serum blood biochemistry of broilers after dietary minerals (Zn-Cr and organic-Se) supplementation under heat stress conditions. (n = 8).
| Treatments | g/dL | mg/dL | U/L | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Total Protein | Albumin | Glucose | Triglycerides | Total Cholesterol | HDL-Cholesterol | LDL-Cholesterol | Creatinine | Uric Acid | ALT | AST | |
| (A) Effect of Zn-Cr mixture | |||||||||||
| Without Zn-Cr | 3.67 b | 1.84 b | 185.77 a | 185.77 a | 149.32 a | 39.84 b | 99.39 a | 0.54 a | 7.89 a | 5.36 | 229.71 |
| With Zn-Cr | 4.03 a | 2.06 a | 153.33 b | 153.33 b | 135.86 b | 42.91 a | 81.05 b | 0.45 b | 6.26 b | 5.28 | 225.30 |
| SEM | 0.03 | 0.02 | 2.93 | 2.93 | 1.88 | 0.66 | 1.25 | 0.01 | 0.06 | 0.05 | 1.80 |
| p-value | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 0.0040 | <0.0001 | 0.0004 | <0.0001 | 0.2712 | 0.1416 |
| (B) Effect of organic Se levels | |||||||||||
| Without Se | 3.85 | 1.99 | 175.30 | 174.10 a | 144.76 | 40.30 | 94.73 a | 0.51 | 7.32 a | 228.27 | 5.33 |
| With LSe ≠ | 3.86 | 1.94 | 168.39 | 163.51 b | 138.01 | 41.65 | 86.58 b | 0.49 | 6.97 b | 227.10 | 5.35 |
| With HSe ≠≠ | 3.84 | 1.92 | 164.96 | 172.73 a | 145.00 | 42.18 | 89.35 b | 0.49 | 6.95 b | 227.90 | 5.28 |
| SEM | 0.04 | 0.03 | 3.59 | 2.96 | 2.31 | 0.81 | 1.53 | 0.02 | 0.08 | 2.21 | 0.06 |
| p-value | 0.9558 | 0.2571 | 0.1453 | 0.0424 | 0.0771 | 0.2607 | 0.0047 | 0.7412 | 0.0045 | 0.7213 | 0.9291 |
| (A × B) Effect of interaction between Zn-Cr mixture and organic Se levels | |||||||||||
| T1 | 3.70 b | 1.85 cb | 195.56 a | 190.29 a | 156.63 a | 39.56 b | 109.45 a | 0.55 a | 8.08 a | 230.42 | 5.40 |
| T2 | 4.01 a | 2.12 a | 155.05 c | 157.92 b | 132.89 c | 41.03 b | 80.01 d | 0.46 bc | 6.55 c | 226.12 | 5.26 |
| T3 | 3.62 b | 1.79 c | 186.79 ab | 178.70 a | 145.81 b | 40.41 b | 96.83 b | 0.52 ab | 7.95 ab | 230.03 | 5.41 |
| T4 | 3.69 b | 1.89 cb | 174.96 b | 185.76 a | 145.50 b | 39.54 b | 91.88 cb | 0.55 a | 7.64 b | 228.68 | 5.28 |
| T5 | 4.09 a | 2.09 a | 150.00 c | 148.32 b | 130.21 c | 42.88 ab | 76.32 d | 0.46 bc | 5.99 d | 224.16 | 5.29 |
| T6 | 4.00 a | 1.96 b | 154.95 c | 159.70 b | 144.50 b | 44.82 a | 86.82 c | 0.42 c | 6.25 cd | 227.11 | 5.29 |
| SEM | 0.06 | 0.04 | 5.08 | 4.19 | 3.26 | 1.14 | 2.16 | 0.02 | 0.11 | 3.12 | 0.09 |
| p-value | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 0.0002 | 0.0258 | <0.0001 | 0.0092 | <0.0001 | 0.7385 | 0.7009 |
a,b,c,d = means with different letters in the same column are significantly different at (p < 0.05). SEM, standard error of the mean. ≠ LSe = low selenium concentration, ≠≠ HSe = high selenium concentration. T1 = (basal diet, no additive), T2 = T1 + 100 mg Zn + 1.5 mg Cr/Kg diet (Zn-Cr), T3 = T1 + 0.6 mg Se/Kg, T4 = T1 + 0.9 mg Se/Kg, T5 = T1 + (100 mg Zn + 1.5 mg Cr + 0.6 mg Se) and T6 = T1 + (100 mg Zn + 1.5 mgCr + 0.9 mg Se).