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. 2023 Jul 26;12(15):1937. doi: 10.3390/cells12151937

Figure 3.

Figure 3

Tr-OxPLs exacerbate HKSA-induced lung injury in two-hit model of ALI. Young and old mice were co-treated with HKSA (intranasal, 4 × 108 bacterial particles) and either KOdiA-PC, Paz-PC, or PONPC (10 mg/kg, i.v., 24 h). (A) BAL was collected to measure total cell counts and protein content; * p < 0.05 vs. HKSA alone, n = 6. In KOdiA-PC/HKSA model, BAL cell counts (13.11 +/− 3.67 vs. 20.78 +/− 7.78 cells × 105, ** p < 0.04 Y vs. O) and protein concentration (857.39 +/− 354.39 vs. 1303.22 +/− 468.53 µg/mL, ** p < 0.02 Y vs. O) were compared between young and old groups. In Paz-PC/HKSA model, BAL cell counts (14.49 +/− 3.56 vs. 19.87 +/− 5.80 cells × 105, ** p < 0.04 Y vs. O) and protein content (691.62 +/− 181.92 vs. 1291.49 +/− 435.79 µg/mL, ** p < 0.03 Y vs. O) were compared. In PONPC/HKSA model, BAL cell counts (12.53 +/− 6.38 vs. 18.97 +/− 6.36 cells × 105, Y vs. O) and protein content (828.23 +/− 328.31 vs. 1189.52 +/− 318.95 µg/mL, ** p < 0.03 Y vs. O) were compared. ND, no difference. (B) Vascular leak was determined using Evans blue extravasation assay; representative lung images of three independent experiments are presented.