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. 2023 Jul 26;12(15):1937. doi: 10.3390/cells12151937

Figure 4.

Figure 4

Lung ECs from old mice develop more pronounced inflammation in response to HKSA or Tr-OxPLs. (A) Isolated lung ECs from young and old mice were exposed to HKSA (108 cells/mL, 6 h) followed by qPCR analysis of senescence marker p21Cip−1 and inflammatory markers VCAM-1, ICAM-1, IL-6, CCL2, TNF-α, KC, and IL-1β expression levels. Data presented as fold-increase over control; n = 3, * p < 0.05 vs. young group. (B) Lung ECs from young and old mice were exposed to indicated Tr-OxPLs (40 µg/mL), and mRNA transcript levels of selected endothelial inflammatory genes were determined by qPCR; n = 3, * p < 0.05 vs. young group. Data expressed as fold increase over control. (C) Both sets of ECs were subjected to HKSA (30 min), followed by the addition of indicated Tr-OxPLs (6 h). qPCR analysis of mRNA expression levels of indicated inflammatory genes; n = 3, * p < 0.05 vs. young group.