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. 2023 Aug 7;15(15):4001. doi: 10.3390/cancers15154001

Figure 1.

Figure 1

Experimental design and structure of agents tested. (A) Chemical structures of chemopreventive agents tested—DFMO, Sulindac, and NO-Sulindac. (B) Experimental protocol for the evaluation of chemopreventive efficacy of DFMO, Sulindac and NO-Sulindac in a rat colon cancer model. Colon tumor efficacy assay: Groups of rats (36 rats/group; 30 AOM + 6 Vehicle) were fed the control diet (AIN 76A) at age 7 weeks; rats were given 15 mg azoxymethane/Kg body weight once weekly for 2 weeks. Thirteen weeks after second AOM injection (adenoma stage), rats were fed the control diet and experimental diets containing 500 ppm DFMO, 150 ppm Sulindac, 200 ppm NO-Sulindac, and combinations of 500 ppm DFMO plus 150 ppm Sulindac or 500 ppm DFMO plus 200 ppm NO-Sulindac for 36 weeks to assess colorectal adenocarcinomas (detailed information has been given in Section 2).