FIG. 3.

HCF-2 is expressed in multiple tissues and is abundant in the testis. Northern blot analysis of HCF-2 and HCF-1 mRNA. Poly(A)⁺ RNA (2 μg) from normal human tissue was probed with an HCF-2-specific probe (A); the blot was then stripped and reprobed with an HCF-1-specific probe (B) or a GAPDH-specific probe (C). The tissues analyzed were spleen (lane 1), thymus (lane 2), prostate (lane 3), testis (lane 4), ovary (lane 5), small intestine (lane 6), mucosal lining of the colon (lane 7), and peripheral blood leukocytes (lane 8). Positions of size markers are shown to the left of each panel.