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. 2023 Jul 27;15(15):3810. doi: 10.3390/cancers15153810

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Assessment of DoTc2 4510 cell dependence on HPV16 E6 and E7 oncoproteins by siRNA knock-down. (a) Expression of HPV16 E6, E7, and p53 analyzed by Western blot after siRNA knock-down. siNeg: siRNA knock-down negative control, Vinculin, loading control. (b) Analysis of cell morphology and detection of senescence-associated β-galactosidase activity after transfection with non-targeting siRNA (siNeg) and HPV16 E6/E7-targeting (siE6/E7) siRNA pools. (c) Colony formation capacity of DoTc2 4510 cells was analyzed after transfection with non-targeting siRNA and HPV16 E6/E7-targeting siRNA pools. Original Western blots are presented in Figure S3.