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. 2023 Jul 31;12(15):1973. doi: 10.3390/cells12151973

Figure 1.

Figure 1

DCUN1D1 is upregulated in prostate cancer cell lines and prostate cancer tissue samples. (a) RT-PCR analysis of DCUN1D1 in prostate cancer cell lines. Total RNA was collected from PREC (normal), DU145, DUCaP, PC3, CL1 (androgen independent) and CW22, CW19, LNCaP and VCaP (androgen dependent cells). Normalization of each sample was carried out by measuring the amount of human GAPDH cDNA. (b) Western blot analysis of DCUN1D1 expression in the prostate cancer cell lines. DCUN1D1 expression was probed with anti-DCUN1D1 antibody. Normalization of each sample was carried out by β-tubulin antibodies. (c) Immunohistochemical staining of DCUN1D1 in representative prostate cancer cells (arrowhead) of tumour tissue exhibited intense expression compared to adjacent normal prostate tissue (long arrow). On the left, staining of DCUN1D1 in normal and prostate cancer tissue (Magnification 100×). On the right, DCUN1D1 immunohistochemical staining of prostate cancer tissue (Magnification 200×). Experiments were independently repeated three times and a representative image of an independent experiment is represented.