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. 2023 Jul 31;12(15):1973. doi: 10.3390/cells12151973

Figure 2.

Figure 2

Blockage of DCUN1D1 expression inhibits proliferation and migration and induces apoptosis in prostate cancer cell lines. Prostate cancer DU145 and PC3 cell lines were infected with lentivirus-encoding shRNA against GFP and DCUN1D1. (a) Real-time PCR analysis of DCUN1D1 in prostate cancer cell lines 48 h post-infection. Total RNA was collected from DU145 and PC3 cell lines and normalized by measuring the amount of glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-complementary DNA. (b) Western blot analysis of DCUN1D1 expression in DU145 and PC3 cell lines 48 h post-infection. DCUN1D1 expression was probed with anti-DCUN1D1 antibody and normalized by measuring the amount of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (c) Proliferations of DU145 and PC3 48 h post-infection. Data means ± s.d. of triplicate independent experiments. (d) Migrations of DU145 and PC3 cell lines were measured 48 h post-infection in transwell plates. Migrating cells were fixed, stained and 3–5 random microscopic fields were counted. Values shown are mean ± s.d. from a representative experiment. (e) Apoptosis analysis of DU145 cell lines post-infection. The Cell Death Elisa Plus kit was used to quantify apoptosis 24 h post-seeding of the cells. Data means ± s.d. of triplicate independent experiments. * p < 0.05, two-tailed Student’s t-test. Experiments were independently repeated three times and a representative image of an independent experiment is represented.