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. 2023 Aug 5;12(15):2005. doi: 10.3390/cells12152005

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Preservation of SR-1 against H₂O₂-induced oxidative stress in EPCs. (A) mRNA levels of AhR, CYP1A1, CYP1A2, and CYP1B1 were examined using qRT-PCR. The mRNA levels were normalized to GAPDH. (B) ROS production measured using flow cytometry analysis using H₂DCFDA dye. (C) Percentages in the representative histograms indicated the percentage of ROS levels. (D) Representative images of γH2A.X (Red) and DAPI (Blue) staining in EPCs (scale bar, 40 μm). The boxed regions are shown at a high magnification (×2) in the inset. (E) γH2A.X foci number per nucleus. (F) The protein expression of γH2A.X were analyzed via Western blotting. The data are presented as mean ± standard deviation. (n.s represents no significance, * p < 0.05 and *** p < 0.001 vs. control, SR-1 1 μM treatment, H₂O₂ 800 μM treatment for 30 min).