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. 2023 Aug 4;12(15):2000. doi: 10.3390/cells12152000

Figure 4.

Figure 4

Nicotine increased migration through nAChRs in the human prostate cancer cell line DU145. Analysis was performed using a label-free impedance-based migration assay (xCELLigence). (A) DU145 cells were treated with 0.1 μM, 1 μM, or 10 μM nicotine in the presence or absence of the inhibitors MEC, MLA, or DHBE. The cell migration index is plotted versus time. (B) Bar diagram of the data (mean ± SEM) at 12 and 24 h from the experiments in A. Measurements were made using 4–5 independent experiments performed in triplicate. Statistical significance was analyzed using the Kruskal–Wallis test for non-parametric data with the GraphPad Prism (GraphPad 9.1.1 Software) software. A p-value of <0.05 was considered significant. * p ≤ 0.05, ** p ≤ 0.001.