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[Preprint]. 2023 Aug 2:2023.07.31.551324. [Version 1] doi: 10.1101/2023.07.31.551324

Figure 1: CCT and the TORC1 pathway promote dendritic arborization.

Figure 1:

(A) Schematic diagram of regulatory relationships between the insulin pathway, SCF complex, CCT, and TORC1 pathway with the insulin pathway indicated by teal arrows. TORC1 is negatively regulated by Cullin1 and positively regulated by CCT. The upstream insulin pathway in green is displayed for context but was not examined in this study. Individual components of the SCF and TORC1 complexes examined in this study are outlined in white. (B) Representative images of CIV neurons for key CCT and TORC1 pathway manipulations, with RNAi-mediated knockdown indicated with -IR and UAS-mediated overexpression with -OE. Scale bars = 100 μm (C) Total dendritic length of CCT and TORC1 pathway manipulations shown in comparison to a WT control, * indicates a significant change from control (p < 0.05). (D) Number of Sholl intersections mapped by color at increasing radial distances from soma (μm). Significant changes in Sholl maximum intersections and radius are indicated by an asterisk. Arrows indicate genotypes where the radius of maximum intersections has shifted significantly from WT. The white dashed line is to reference the radius of maximum intersections for WT neurons. In all panels * = p < 0.05, see Supplementary Table S2 for detailed statistics.