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[Preprint]. 2023 Jul 31:2023.07.31.551186. [Version 1] doi: 10.1101/2023.07.31.551186

Figure 2. MRE11 nuclease inhibition leads to increases in C-circles and C-rich ssDNAs.

Figure 2.

(A) C-circle assay for U2OS and SaoS2 cells with or without Mirin (MRE11 nuclease inhibitor) treatment. Samples were fractionated into nuclear, and cytoplasmic DNA fractions. U2OS and Saos2 cells were treated with 50 μM Mirin for 48 hr. (B-C) Quantification of the C-circle assay in A; U2OS cells (B), and SaoS2 cells (C), as the relative amount of C-circle assay products (mean ± SD; unpaired t-test). (D) 4SET assay for U2OS and SaoS2 cells with or without Mirin treatment after fractionation of samples into Whole, Nuclear, and Cytoplasmic DNA fractions. G-rich probe was used to detect C-rich telomeric sequences, and C-rich probe was used to detect G-rich telomeric sequences. C-rich single stranded DNAs are indicated by an arrow.