FIG. 4.
HHV-6 dramatically suppresses HIV replication in coinfected DC cultures. (A and B) Purified DC were exposed overnight to HHV-6Z29 at an MOI of 0.1, washed, placed back into culture for 2 days, and exposed to HIVBaL (A) or HIVIIIB (B) at an MOI of 0.1 (arrows). (C and D) Purified DC were exposed overnight to HIVBaL (C) or HIVIIIB (D) at an MOI of 0.1, washed, placed back into culture for 2 days, and exposed to HHV-6Z29 at an MOI of 0.1 (arrows). Culture supernatants were assessed for HIV-1 p24 content every other day by RIA. The results shown are representative of at least five separate experiments. □, HIV alone; ▵, coinfection; ○, HHV-6 alone.