FIG. 5.

Pattern of HIV replication in PHA-stimulated PBMC and macrophages coinfected with HHV-6. PBMC were activated with PHA for 3 days prior to infection and maintained in media containing IL-2 following infection. Macrophages were propagated from elutriated monocytes cultured for 7 days in the presence of GM-CSF and maintained in media containing GM-CSF following infection. (A to C) PHA-stimulated PBMC (A and B) or macrophages (C) were exposed overnight to HHV-6_Z29 at an MOI of 0.1, washed, placed back into culture for 2 days, and exposed to HIV_BaL (A and C) or HIV_IIIB (B) at an MOI of 0.1 (arrows). (D to F) PHA-stimulated PBMC (D and E) or macrophages (F) were exposed overnight to HIV_BaL (D and F) or HIV_IIIB (E) at an MOI of 0.1, washed, placed back into culture for 2 days, and exposed to HHV-6_Z29 at an MOI of 0.1 (arrows). Culture supernatants were assessed for HIV-1 p24 content every other day by RIA. The results shown are representative of at least three separate experiments. □, HIV alone; ▵, coinfection; ○, HHV-6 alone.