FIG. 7.
Infectious HHV-6 is required to suppress HIV-induced syncytium formation in coinfected DC cultures. (A) Purified DC were exposed overnight to HIVBaL at an MOI of 0.1, washed, and placed back into culture. (B to D) Purified DC were exposed overnight to HHV-6Z29 at an MOI of 0.1, washed, placed back into culture for 2 days, and exposed to HIVBaL at an MOI of 0.1. (C and D) DC were exposed to HHV-6 that had been preincubated with 0.5 (C) or 500 (D) μg of neutralizing anti-HHV-6 MAbs per ml for 1 h at 37°C. Photographs of culture dishes were taken 14 days following infection and show numerous large HIV-induced syncytia (arrows) in panels A (no HHV-6) and D (neutralized HHV-6).