FIG. 8.
HIV does not affect HHV-6 replication in coinfected DC cultures. Purified DC were exposed overnight to HHV-6Z29 at an MOI of 0.1, washed, placed back into culture for 2 days, and exposed to HIVBaL at an MOI of 0.1 (arrows). (A) Culture supernatants were assessed for infectious HHV-6 content every other day by a plaque assay with PHA-stimulated PBMC as targets. Values represent means and standard deviations in triplicate cultures. (B) Culture supernatants were assessed for HIV-1 p24 content every other day by RIA. The results shown are representative of at least three separate experiments. □, HIV alone; ▵, coinfection; ○, HHV-6 alone. TCID50, 50% tissue culture infective dose.