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[Preprint]. 2023 Aug 2:2023.08.02.551708. [Version 1] doi: 10.1101/2023.08.02.551708

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Fig 1 — CRISPRi screening overview. (A) Design and construction of a Mobile-CRISPRi library targeting all putative essential genes in A. baumannii 19606. The library was screened with CRISPRi inducer (1 mM IPTG) to identify genes that are vulnerable to knockdown or with inducer and a sub-MIC concentration of antibiotic to identify antibiotic-gene interactions. (B-C) Density plot showing depletion of essential gene targeting sgRNA spacers (perfect match or mismatch) from the library but not depletion of non-targeting control sgRNAs during growth over two time points (T₁ and T₂) (D) The population diversity (N_b) of essential gene targeting sgRNAs is reduced relative to controls, indicating that those sgRNAs are depleted during growth. The white horizontal line through the bars indicates a break in the data.