Fig 7.

COL/RIF interaction with and physiological characterization of NDH-1 knockdown. (A) CoMBaT-seq data from a growth competition between a nuoB knockdown strain and a non-targeting control strain in the presence or absence of COL. Only data from the gene targeting strain is depicted as the non-targeting control is the remaining proportion of the population. Points are data from individual experiments (N = 2). (B) Measurement of the NAD+/NADH ratio in nuoB knockdown and non-targeting cells using the NAD/NADH-Glo assay. An unequal variance t-test was performed and the asterisk indicates that the p value ≤ 0.05. (C-D) Knockdown-response curves of nuoB show a nearly monotonic response in the absence of RIF, but a hormetic response in the presence of RIF. (E) CoMBaT-seq data from a growth competition between a nuoB knockdown strain and a non-targeting control strain in the presence or absence of RIF. (F) EtBr permeability assay of non-targeting and nuoB knockdown strains; nuoB knockdowns show decreased access of EtBr to DNA in the cytoplasm.