FIG. 6.

HHV-8 DNA load in pBMC from an AIDS-KS patient before (day 0) or after (day 6) culture in the presence (αIFN) or absence (RPMI) of IFN-α2b (100 IU/ml). Shown is the autoradiogram of a serial-dilution PCR experiment performed with primers specific for HHV-8 ORF 72 (v-cycD) (A) or for the human β-globin gene (B). PCR products were hybridized with specific oligonucleotide probes. Numbers above the lanes represent dilution factors (A) or aliquots of cell extracts corresponding to the indicated number of cells (B). NC, negative control made with salmon sperm DNA; PC, positive-control PCR performed with the indicated number of the control plasmid pCRII-v-cycD (A) or 250 pg of human DNA from a uterine cervical carcinoma cell line (B).