FIG. 7.

ISH with an antisense riboprobe for T0.7 mRNA (bright-field results on the left, dark-field results on the right) of PBMC from an AIDS-KS patient after culture with anti-IFN-α Ab. (Panels 1) PBMC from the AIDS-KS patient cultured for 7 days in the presence of anti-IFN-α. The arrow points to a cell showing T0.7 signals. Cells were negative after ISH with a VP23 antisense riboprobe (data not shown). (Panels 2). Negative control made with Jurkat cells. (Panels 3). TPA-induced BCBL-1 cells hybridized with a VP23 antisense probe as a control of the ability of ISH to detect VP23 expression under the conditions used. (Panels 4) TPA-induced BCBL-1 hybridized with the T0.7 probe. (Panels 5) PBMC from the AIDS-KS patient hybridize with a probe for β-actin mRNA. No T0.7 signals were observed in the adherent cells from the patient after culture without anti-IFN-α Ab (data not shown). PBMC were negative after ISH with a VP23 antisense riboprobe (data not shown).