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[Preprint]. 2023 Aug 4:2023.08.02.551695. [Version 1] doi: 10.1101/2023.08.02.551695

Figure 2: Neuronal cilia staining in hypothalamic feeding centers show ARL13B is usually in cilia.

Figure 2:

(A-F) Representative immunofluorescence staining of neurons in the hypothalamic feeding centers (PVN, VMH, ARC) in the mouse brain stained with antibodies against ACIII (red) and ARL13B (green). Scale bar for large panels: 20um; scale bar for insets: 5um. (G-H) Representative RNAscope in situ hybridization images of AgRP (red) and POMC (blue) neurons in the ARC. (I) Quantification of POMC neurons (n = 3 per group). Scale bar 100 μm. Error bars represent standard deviation.