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[Preprint]. 2023 Aug 4:2023.08.03.551815. [Version 1] doi: 10.1101/2023.08.03.551815

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Figure 6. — (A, B) Example confocal images (Z-projections) of mitotic spindles in live control and (A) S3372C and (B) S3372C/+ embryos with fluorescently-labeled dynein (GFP-Dlic). Embryos additionally contain markers of kinetochores (Spc25-RFP; A) and chromatin (Histone H2A-RFP; B). In B, arrow and arrowhead show examples of accumulation of dynein at the metaphase plate in S3372C/+ embryos. Ectopic GFP-Dlic accumulation at ≥ 1 kinetochore was observed in 100% (21/21) of S3372C embryos, 43% (10/23) of S3372C/+ embryos and 0% (20/20) of control embryos. Scale bars: 10 μm.