Figure 8. Fibrous bundles observed by cryo-FIB and cryo-ET in in E. coli cells expressing INP.

A) Ion-beam image of a thin lamella containing E. coli cells expressing INP obtained from cryo-FIB milling. B) Zoomed-in view of a cryo-TEM image of the lamella in A). Boxes with dashed-lines indicate areas where tilt series were collected. C) and D) Snapshots from 3-D cryo-tomograms reconstructed from tilt series collected in the boxed regions in B) showing striking fibrous bundles (yellow arrowheads). The E. coli cell envelopes are indicated with thick dash-lines. E) Further examples of the fibrous bundles produced by INP-expressing E. coli. Size markers in A) is 10 μm, in B) is 2 μm and in C), D) and E) are 100 nm, respectively.