Fig 6. ROC curves comparing gene rankings in MAGeCK and CRISPRi-DR for enriched hits (A) and depleted hits (B) in 1 day pre-depletion experiments.

The recovery of the depleted hits outperforms the recovery of enriched hits, showing that MAGeCK and the CRISPRi-DR model rank depleted genes similarly. EMB and STR are excluded in the ROC analysis of depleted genes and CLR and VAN are excluded in the analysis of enriched genes. These libraries had too few significant genes reported by MAGeCK in their respective categories to yield meaningful ROC curves. The lower performance of the enrichment gene rankings may be due to a few reasons, including noise.