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[Preprint]. 2024 Feb 7:2023.08.03.551759. Originally published 2023 Aug 5. [Version 2] doi: 10.1101/2023.08.03.551759

Fig 1. Effect of sgRNA efficiency on concentration dependence for sgRNAs in rpoB in a CRISPRi library treated with RIF (D5).

Fig 1.

(A) Regression lines for log(relative abundance) against log(concentration) for all sgRNAs in rpoB in a library treated with RIF for 5 days pre-depletion. The lines that reflect the extremes of the sgRNA efficiency (red or purple), are flat and do not show much change in abundance. Comparatively, intermediate sgRNA efficiency (dark green to indigo) shows the most negative slopes, reflecting maximum synergy with drug. (B) Comparison of sgRNA efficiency and slopes of the regressions seen in Panel A for each sgRNA. Each point is an sgRNA colored by its efficiency. The most efficient sgRNAs (purple) and the least efficient sgRNAs (red) show concentration slopes around 0. The dotted line reflects the minimum of the parabolic curve. (C) Histogram of sgRNA efficiencies where the slopes reach their most extreme (positive or negative) for 236 interacting genes in RIF D5. The distribution shows that most of the extrema sgRNAs for interacting genes fall in the range of −5 to 0 (note: not the strongest sgRNAs, which would have efficiencies around −25).