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. 2023 Aug 2;24(15):12360. doi: 10.3390/ijms241512360

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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MTT cell viability assay. Canine Progenitors Epidermal Keratinocytes (CPEK) were untreated or treated with formulated or non-formulated probiotic bacteria at various concentrations for 24 h, and cell viability was determined using a colorimetric MTT assay. The results revealed that both the formulated and non-formulated probiotic bacteria were well tolerated by CPEK cells, except at very high concentrations. Here, the control (untreated) group is referred to as 100% viable cells. Data are presented as the mean ± standard deviation of one experiment conducted in duplicate (N = 2). * indicates (p < 0.05).