Skip to main content
. 2023 Jul 28;24(15):12121. doi: 10.3390/ijms241512121

Figure 3.

Figure 3

Effects of ZSCAN4 KD on histone modification and ZGA. (A) Western blotting was used to determine H3K9me3 level in the four-cell stage. (B) Western blotting was used to determine H3K27ac level in the four-cell stage. (C) Embryos in the four-cell stage were immunolabeled with anti-H3K9me3 (green); Hoechst 33342 was used to label DNA (blue). Bar = 20 μm. (D) Staining images of H3K27ac in control four-cell embryos (n = 71) and ZSCAN4 KD four-cell embryos (n = 67). (E) qRT-PCR assay was used to determine the mRNA level of eIF1a, Tbx3, Tcstv3, Rif1, Wee1, and Dppa2 in the four-cell stage. Green, H3K27ac; blue, DNA. Bar = 20 μm. Results with * p < 0.05, ** p < 0.01 and *** p < 0.001 were considered significant.