Figure 6.
Effects of VaM on breast cancer cell growth in a xenograft mouse model. (A) Schematic representation of the experimental methods described in the Materials and Methods section. MCF-7 cells (5 × 106 cells/mouse) were mixed with Matrigel (1:1, v/v) and injected subcutaneously. Mice were randomly assigned to 6 groups and intraperitoneally administered 100 μL of PBS or each drug (25 and 50 mg/kg of VaM and 20 mg/kg of doxorubicin) 3 times a week for 14 days. The tumor volume was measured, and the mice were euthanized at the end of the treatment. (B) Changes in body weight and (C) tumor volume were monitored for 2 weeks after drug administration. (D) Representative photographs of harvested tumors from each treatment group. (E) Tumor weight was measured. (F) Immunoblotting analysis of tumor tissue lysates for p-STAT3, STAT3, cleaved-PARP, and pro-caspase 3 protein expression levels. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared to the no treatment concentration group. ### p < 0.001 compared to VaM H group. N.S means no significant difference.