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Subcellular localization and transcriptional activation analysis. (a) Localization of BcNF-Ys within the cells of Nicotiana benthamiana was examined. (b) Schematic illustration for transcriptional activity assay. (c) The relative luciferase (LUC) activities observed in Nicotiana benthamiana leaves indicate the transcriptional activation potential of BcNF-Y proteins. ‘**’ indicates p-value < 0.01, and ‘***’ indicates p-value < 0.001, by one-way ANOVA analysis with Tukey’s HSD test.
