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. 2023 Jul 27;24(15):12017. doi: 10.3390/ijms241512017

Figure 1.

Figure 1

Distribution of the different airway epithelial cell types in the three lung tissue models. (A) Schematic representation of the scRNA-seq experimental workflow: airway epithelium sources (biopsy/brushing-derived cells, ALI culture of primary bronchial epithelial cells and iALI culture of iPSC-derived epithelial-like cells), generation of scRNA-seq libraries and sequencing, computational analysis to identify cell types. The brushing/biopsy epithelium scRNA-seq data were from [24]. The ALI and iALI scRNA-seq data were generated in our laboratory (see Methods); (B) contribution of each cell type in the three models. UMAP and tSNE were used to show the contribution of SCGB1A1+, TP63+, FOXJ1+, FOXI1, POU2F3+ and ASCL1+ cells (dark gray). (C) UMAP projection of the different cell types. The feature plots display the subset of airway epithelial cells obtained from biopsy/brushing of healthy donors [24]. (D) Maps of the expression of ssRNA virus receptors (rhinovirus C receptor (CDHR3), Coxsackievirus B1 receptor (CD55) and SARS-CoV-2 receptor (ACE2)) in single cells from the three models. UMAP and tSNE were used to show the cell types that express the ACE2, CDHR3 and CD55 receptors.