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. 2023 Aug 4;24(15):12427. doi: 10.3390/ijms241512427

Figure 2.

Figure 2

CC5 and CC8 reduce cell adhesion using cell adhesion assay. (A) Effect on THP-1-derived macrophage. (a) Cells were incubated without (black bars), or with 100 nM CC5 (dark grey bars), or with 100 nM CC8 (light grey bars) for 30 min. Cells were then allowed to attach on fibrinogen (Fg), Collagen I (Coll I), vitronectin (Vn), or poly-L-lysine (PLL). (b) Cells were stimulated with LPS (1 µg/mL) and seeded in wells coated with different extracellular matrices and incubated without or with various concentrations of CC5. (c) LPS-stimulated THP-1 cells were seeded in wells pretreated with different extracellular matrices and incubated without or with various concentrations of CC8. Non-treated cells were used as negative control. (B) Effect on RAW264.7 cells. (a) Cells were incubated without (black bars), or with 100 nM CC5 (dark grey bars), or with 100 nM CC8 (light grey bars) for 30 min. Cells were then allowed to attach on Fg, Coll I, Vn, or PLL. (b) Cells were stimulated with LPS (1 µg/mL), seeded in wells coated with different extracellular matrices and incubated without or with CC5 at various concentrations. (c) LPS-stimulated RAW264.7 cells were seeded in wells pretreated with different extracellular matrices and incubated without or with various concentrations of CC8. Non-treated cells were used as negative control. Data are representative of three independent experiments performed in triplicate, expressed as mean (±SD). p < 0.05 was considered statistically significant and is indicated with asterisks over the value (* p < 0.05, ** p < 0.01, *** p < 0.001).