FIG. 3.

The GFP-22 virus replicates as efficiently as the WT virus. Vero cells infected with either the WT virus or 166v at 10 PFU per cell were harvested every 3 h after infection, up to 24 h, as indicated above the gels. Equal amounts of total cell lysates were analyzed by SDS-PAGE followed by Western blotting with antibodies against VP22 and GFP (A); IE110, TK, and VP16 (B); or α tubulin and acetylated tubulin (C).