Figure 5.

Flow cytometric gating strategy for blood immune cells bound to fPS-MP microplastics. (A) Total CD45⁺ leukocytes (black box) and fPS-MPs binding leukocytes (red box). (B) Separation of the population of CD45⁺ leukocytes in the black box of panel A into lymphocytes (Lym), granulocytes (Gran), and CD11b⁺ monocytes (Mono) (black boxes). (C–F) Each population was separated from B using the following markers: (C) Gr-1⁺ neutrophils (black box); (D) CD8⁺ T cells (black box); (E) CD4⁺ T cells (black box); (F) CD11b⁺ monocytes (black box). In (A–F), the red boxes indicate co-labeling for fPS-MP microplastic and marker-specific positive cells.