Figure 3.

The role of PKCα on PREP1-mediated expression of proinflammatory cytokines and antioxidant molecules. CTRL and Prep1 overexpressed in MAEC cells were incubated with bisindolylmaleimide (BIM) at a 100 nM concentration for 30 min. Tnf-α (a), Il-6 (b), Il-1β (c), Sod2 (d), and Atf4 (e) mRNA levels were evaluated by real-time RT-PCR analysis in CTRL and Prep1 overexpressing MAEC cells. Data were normalized by the amount of beta-actin used as an internal control. Bars represent the mean ± SD of three independent experiments, each performed in triplicate. Asterisks denote statistical differences (* p < 0.05; ** p < 0.01; *** p < 0.001).