Figure 4.

The role of resveratrol on PREP1-mediated eNOS signaling. CTRL and Prep1 overexpressed in MAEC cells were incubated with resveratrol (RSV) at a 20 μM concentration for 2 h. The protein expression of PREP1, pPKCα, PKCα, peNOS^Thr495, and eNOS was analyzed by Western Blot using ACTIN antibody for normalization. The autoradiographs shown are representative of three different experiments and subjected to densitometric analysis. Bars represent the mean ± SD of three independent experiments, each performed in triplicate. Asterisks denote statistical differences (* p < 0.05; ** p < 0.01; *** p < 0.001).