Figure 3.

Evaluation of two types of silica particles as RNA adsorbent: mesoporous silica and spherical silica. The LiCl-precipitated IVT RNA (2 μg) mixed with spermidine at various N/P ratios was purified by a spin column filled with mesoporous silica or spherical silica using an elution buffer containing 300 mM NaCl. (A) Binding and elution of the IVT RNA using mesoporous silica or spherical silica in the presence of spermidine at an increasing N/P ratio. Aliquots of the fractionated RNA samples were analyzed on 1.0% agarose gel stained with GelRed and visualized by UV illumination. (B) The relative amounts of RNA present in each flowthrough (unbound RNA) and eluant (silica-bound RNA), which are shown in agarose gel (A), were quantified and normalized to 1 μg RNA loaded in the separate lane.