Table 2.

Simulation of the release of intermediate peptides.

Intermediate Peptide	Type of Demasking	Hydrolysis Rate Constants (min−1)	Calculated Values of dr (%) 1	Experimental Estimation of dr 1 (%)
f(9–69/70), ABC	One-stage 2	k14= 0.53, k40 = 1.41	2.6	1.5 3
f(9–40), AB	One-stage		3.5	3.6
f(15–69/70), BC	One-stage		2.8	- 4
f(76–100/101) ABC	Two-stage 5	k83= 1, k91 = 1	3.9	3.4
f(76–91), AB	Two-stage		4.3	4.4
f(84–100/101), BC	Two-stage		4.3	4.7
f(101/102–138), ABC	Two-stage	k124= 2, k138 = 0.2	3.8	3.4
f(101/102–135), AB	Two-stage		4.1	- 4
f(125–138), BC	Two-stage		4.7	6.1

¹ The average degree of hydrolysis was determined by Equation (8). ² For the one-stage demasking, $k_d^f$ was 0.46 min⁻¹ [18]. ³ This fragment was found only at one degree of hydrolysis d = 1.5% [9]. ⁴ These peptide fragments were not found [9]. ⁵ For the two-stage demasking, k_d was 0.15 min⁻¹ ($k_d^f$/k_d = 3 [18]).