Table 3.

Simulation of the release of final peptides.

Final Peptide	Type of Demasking	Hydrolysis Rate Constants (min−1)	Calculated Values of n 1	Experimental Estimation of n 1
f(9–14), A	One-stage 2	k14= 0.53, k40 = 1.41	0.85	0.68
f(15–40), B f(41–69/70), C	One-stage One-stage		0.92 0.44	0.86 0.59
f(76–83), A	Two-stage 3	k83= 1, k91 = 1	2.41	1.46
f(84–91), B	Two-stage		2.86	2.37
f(92–100/101), C	Two-stage		2.41	1.40
f(101/102–124), A	Two-stage	k124= 2, k135 = 0.2	2.21	1.13
f(125–135), B	Two-stage		4.84	5.76
f(136–138), C	Two-stage		4.77	5.10

¹ The exponent of the power function n was determined using Equation (9). ² For the one-stage demasking, $k_d^f$ was 0.46 min⁻¹ [18]. ³ For the two-stage demasking, k_d was 0.15 min⁻¹ ($k_d^f$/k_d = 3 [18]).