Overexpression of mir-219. (A) Overexpression of mir-219 has a positive effect on the longevity of Htt.Q25 expressing (control) flies, while a negative effect on the longevity of Htt.Q120 expressing (HD) flies. (B) mir-219 overexpression leads to decreased eclosion rate of both HD and control flies. The graph shows the relative eclosion of flies expressing Htt.Q120 or Htt.Q25 in the presence or absence of a mir-219 transgene as percent of non-expressing siblings. (C) Overexpression of mir-219 leads to a slight decrease in the number of rhabdomeres per ommatidia in control flies, but it does not affect neurodegeneration in the eyes of HD flies. The boxes show 25%, 50%, and 75% values, whiskers represent 10% and 90% values. (D) Overexpression of mir-219 reduces the climbing speed of both HD flies and controls. Bars show average values, error bars indicate SEM. Significance levels (** p ≤ 0.01, *** p ≤ 0.001) are shown only between corresponding mir-219 overexpressing and non-overexpressing categories.