TABLE 3.
RNA packaging for an RNA remaining in the nucleus (measured as a percentage of total RNA produced)
| Plasmid(s)a | Total intensity/per plate
|
% Gag-Pol RNA in virions | |
|---|---|---|---|
| Cellular Gag-Pol RNAb | Virion-associated Gag-Pol RNAc | ||
| pHR1256 + pCMVrev + pCMVtat | 1.68 × 107 | 3.33 × 106 | 16.6 |
| pHR1152 | 8.72 × 105 | 1.05 × 105 | 10.7 |
| pHR1152 + pHR1256 |
1.46 × 106 | 1.52 × 105 | 9.4 |
| 1.74 × 107 | 3.46 × 104 | 0.2 | |
a
Values for pHR1256 alone were not determined since no virion particles were produced.
b
The measured intensities of the bands in the lanes marked C in Fig. 4B were multiplied by a correction factor of 360 as described in Materials and Methods.
c
The measured intensities of the bands in the lanes marked V in Fig. 4B were multiplied by a correction factor of 5 as described in Materials and Methods.