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. 2023 Jul 30;24(15):12217. doi: 10.3390/ijms241512217

Figure 3.

Figure 3

The effect of 24-h pre-incubation of cortical astrocytes with 1 or 5 μg/mL of SeNrs on the OGD/R-induced cell death. (A) Double staining of cells with Hoechst 33342 (HO342), propidium iodide (PI), merge HO342 with PI. OGD/R—induction of OGD (2 h) and reoxygenation (24-h) without preincubation with SeNrs. (B) Cytogram demonstrating the viability of cortical astrocytes after OGD/R (2h and 24-h reoxygenation) and after 24-h pre-incubation with 1 or 5 μg/mL SeNrs and OGD/R. X-axis—the intensity of PI fluorescence; Y-axis—the intensity of Hoechst 33342 fluorescence. Cells were stained with the probes 24-h after the OGD/R. (C) Effect of 24-h pre-incubation with 1 or 5 μg/mL SeNrs on the induction of necrosis and apoptosis. The number of replicates for cell cultures was 3, the number of cover-slips with cells for each sample was 5. Statistical significance was assessed using t-tests. Comparison of experimental groups vs OGD/R: n/s—data not significant (p > 0.05), * p < 0.05, *** p < 0.001. Comparison of experimental groups with each other is indicated in red.