Figure 9.

The influence of miR-143-3p on junction proteins. (A) Hsa_miR_143-3p expression was measured by qRT–PCR. (B) The expression levels of junctional proteins were examined by WB in CV-A10-infected HUVECs. (C) The expression of junctional proteins in HUVECs treated with si-Control vector, CV-A10 infection, and si-miR-143-3p vector plus CV-A10 infection was identified with WB at 24 and 72 hpi. (D) Confocal imaging showed the junctional protein localization of claudin-5, occludin, ZO-1, and VE-cadherin at 72 hpi. RT–qPCR, quantitative reverse transcription–polymerase chain reaction; WB, Western blotting; CV-A10, coxsackievirus A10; HUVECs, human umbilical vein endothelial cells.