| Volume | Final concentration | |
|---|---|---|
| ATP | 1 μL | 5 mM |
| CTP | 1 μL | 5 mM |
| GTP | 1 μL | 5 mM |
| UTP | 1 μL | 5 mM |
| 10x Reaction buffer | 1 μL | 1x |
| RNAse inhibitor (40 U/μL) | 0. 25 μL | 1 U |
| SP6 enzyme mix | 1 μL | 1x |
| Pooled template DNA | 200 ng | 20 ng/ μL |
| Nuclease-free water | to 10 μL |
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