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. Author manuscript; available in PMC: 2023 Aug 11.
Published in final edited form as: Nat Protoc. 2023 Apr 17;18(6):1841–1865. doi: 10.1038/s41596-023-00821-y
Volume Final concentration
10 μM Barcode forward 2.5 μL 0.5 μM
10 μM Barcode reverse 2.5 μL 0.5 μM
5x HF buffer 10 μL 1x
10 mM dNTPs 1 μL 200 μM
DMSO 1.5 μL 3% v/v
Template DNA from Step 15 50 ng 1 ng/μL
Phusion HS Flex polymerase 0.5 μL 1 U
Nuclease free water To 50 μL