Figure 2.

Neural responses of the murine dorsal OB to stimulation with the T&T and the IVO odors. (A) Confocal image of the GCaMP3 signals before odor stimulation. Scale, 500 μm. (B) Schematic of the experimental design. Glomerular activity in the dorsal region of the murine OB was evaluated by imaging using olfactometers. First, the OE was stimulated with a mixture of the five odors used in the T&T test. Next, prosulthiamine was administered via the tail vein (0.2 mg/kg). (C) Left, images of the OB dorsal surface on the right side. g1–g7 are the individual glomeruli (black, glomerulus activated by the T&T odors; red, glomerulus activated by the IVO odor). Scale bar, 500 μm. Right, odorant-evoked Ca²⁺ responses in the target glomerulus. Black bars show the timing of odorant delivery (10 s). (D) Summary of the number of glomeruli activated by the T&T and the IVO odors. There were no significant differences between the number of glomeruli activated by the IVO odor and that activated by the T&T odors. Mann–Whitney test, NS, not significant, n = 5 mice. (E) Summary of the neural responses to stimulation with the T&T and IVO odors (T&T odors, 35 glomeruli; IVO odor, 44 glomeruli). The neural response to stimulation with the IVO odor was significantly greater than that to stimulation with the T&T odors. Mann–Whitney test, *** p < 0.001, n = 5 mice.