Figure 2.
The direct interaction of L1 with LC3 is mediated by the LIR motif LSYHPV in the fourth FNIII domain. (a) Recombinant LC3 was substrate-coated and incubated with increasing concentrations of L1/Fc and CHL1/Fc. (b) Recombinant LC3 was substrate-coated and incubated with L1/Fc, Ig1–6/Fc, and FN1–5/Fc in the absence (-) or presence of a 5-fold excess of the LIRWT or LIRmut peptide. (a,b) Binding was determined by ELISA using HRP-conjugated anti-Fc antibody. Mean values ± SD from three independent experiments carried out in triplicates are shown. **** p < 0.001 relative to the treatment in the absence of peptides, one-way ANOVA with Bonferroni’s multiple comparison test.