Figure 3.
Interaction of L1 with LC3 in cultured cortical neurons is enhanced by function-triggering L1 antibody and reduced by a cell-penetrating peptide containing the extracellular LIR motif LSYHPV. Cultured cortical neurons were treated without (vehicle) or with the tat-LIRWT peptide (tat-LIRWT) followed by treatment without (unstimulated) or with (stimulated) the function-triggering L1 antibody 557 (557). The neurons were fixed and then subjected to proximity ligation with mouse L1 antibody C-2 and a rabbit LC3 antibody. Nuclei are stained with DAPI (blue). (a) In the representative images, red spots indicate the close interaction of L1 and LC3. Scale bars: 10 μm. (b) Mean values + SEM are shown for the average numbers of L1/LC3-positive red spots per cell from three independent experiments (**** p < 0.001 relative to vehicle treatment; #### p < 0.001 relative to 557 treatment; one-way ANOVA with Bonferroni’s multiple comparison test).