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. 2023 May 31;10(7):uhad115. doi: 10.1093/hr/uhad115

Figure 5.

Figure 5

. FvWRKY50 regulated anthocyanin accumulation in strawberry fruit. (A) Anthocyanin accumulation phenotypes of WT and FvWRKY50 CR 2-9 fruits at different developmental stages. Fruits of WT and FvWRKY50 CR 2-9 were harvested and photographed 17, 42, and 47 days after anthesis. (B) Anthocyanin content of WT and FvWRKY50 CR 2-9 (left), EV-OE, and transient OE (middle), and EV-RNAi and transient RNAi (right) fruits. (C) Anthocyanin accumulation-related gene expression levels of WT and FvWRKY50 CR 2-9 fruits 47 days after anthesis. (D) Phenotypes of FvWRKY50-OE and FvWRKY50-RNAi fruits. Octoploid strawberry fruits at big green stage were selected for transient transformation. Control (EV-OE, EV-RNAi) and transient (FvWRKY50-OE, FvWRKY50-RNAi) fruits were photographed 0, 3, 6 and 9 days after injection. (E) FvWRKY50 gene expression levels in EV-OE and FvWRKY50-OE (transient OE) and EV-RNAi, FvWRKY50-RNAi (transient RNAi). qRT–PCR assay was used. (F, G) EMSAs showing that FvWRKY50-His protein could bind to CHI (F) and DFR (G) promoters directly. Negative control is shown in the first lane. Unlabeled probe (200-fold excess) was used as competitor.