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gK is a component of the virion. Protein lysates were run on an SDS–12.5% polyacrylamide gel and transferred to a nitrocellulose membrane. (A) Polyclonal rabbit antiserum αgKN was used as the probe and detected with anti-rabbit antibody conjugated with HRP. A gK-transformed cell line (K9) which expresses cell-associated gK was used as a control. (B and C) The purity of cell-free virus was verified with anti-ORF61 and anti-gE sera.
